Fig. 6.

Impact of SMSr and SMS2 inactivation on CPE and SMS activity in mouse liver and brain. A, B: TLC separation of reaction products formed after incubation of liver and brain microsomal preparations with NBD-Cer. NBD-CPE production in brain is completely abrogated in SMSrD348E and SMSrdelEx6 single mutants, but not in SMS2gt microsomes (A). In contrast, in the liver, production of NBD-CPE is only reduced in single mutants and not detectable in SMS2gt×SMSrD348E and SMS2gt×SMSrdelEx6 double mutants (B). (Data are representative of at least two independent experiments).