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. 2015 Mar 25;11(3):e1004778. doi: 10.1371/journal.ppat.1004778

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Fig 5 — (A) Human MDM from 3 healthy blood donors (1–3) were co-cultured with A. suum larvae in the absence or presence of IS from challenge-A.suum infected pigs and time-lapse movies were recorded (2 frames/ s, 1 min); upper panel: representative snap shots at T = 0; lower panel: representative temporal color code images (120 frames, 1 min)—colors represent different time points; Scale bars 100 μm; (B) Adherent MDM per A. suum larva were counted in time-lapse movies; (C) CXCL3 in culture supernatants from human MDM cultured with or without immune sera from Ascaris-infected pigs (IS), Ascaris suum larvae (A.s.) or both (A.s. IS) quantified by ELISA; (D) Scratch wound closure by human MF cultured in the presence of conditioned media from MDM (MDM sup) stimulated with A.s. IS +/- the CXCR2 antagonist SB265610; All data are pooled from at least 3 independent experiments with n = 3 co-cultures in each and presented as mean +/-SEM.