Table 1. Protein functions dysregulated in NOX1 reduced cells.
Spot | Best homologue | Gene | Accession number | shNOX1 regulation (2DE) | Total score | Matched spectra | Unique peptindes | Sequence coverage | WB validation | |||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Protein name | Abbrev. | Fold | p value a | Fold | p value b | |||||||
1 | Protein SET | SET | SET | Q01105 | 0.5 | 0.008 | 138 | 11 | 5 | 20% | 1.4 | 0.001 |
2 | Protein SET | SET | SET | Q01105 | 0.4 | 0.001 | 91 | 10 | 5 | 22% | ||
3 | Protein SET | SET | SET | Q01105 | 0.3 | 0.003 | 48 | 4 | 3 | 14% | ||
4 | Protein SET | SET | SET | Q01105 | 0.3 | 0.007 | 53 | 5 | 3 | 14% | ||
5 | Methylosome protein 50 | MEP50 | WDR77 | Q9BQA1 | 0.5 | 0.048 | 184 | 17 | 5 | 19% | ||
6 | Methylosome protein 50 | MEP50 | WDR77 | Q9BQA1 | 0.6 | 0.002 | 723 | 59 | 11 | 44% | ||
8 | Proteasome activator PA28 subunit beta | PA28b | PSME2 | Q9UL46 | 0.5 | 0.001 | 453 | 52 | 12 | 41% | ||
9 | Annexin A3 | Annexin A3 | ANXA3 | P12429 | 0.5 | 0.023 | 919 | 100 | 16 | 50% | ||
11 | Hydroxymethylglutaryl-CoA synthase, cytoplasmic | HMG-CoA synthase | HMGCS1 | Q01581 | 0.6 | 0.023 | 843 | 63 | 21 | 49% | 0.5 | 0.007 |
12 | Alpha-fetoprotein precursor | Alpha-fetoprotein | AFP | P02771 | 0.6 | 0.044 | 3672 | 395 | 40 | 75% | 0.6 | 0.037 |
13 | Phosphoenolpyruvate carboxykinase [GTP], mitochondrial | PEPCK-M | PCK2 | Q16822 | 0.5 | 0.007 | 384 | 28 | 9 | 16% | ||
Serum albumin | Serum albumin | ALB | P02768 | 61 | 6 | 5 | 8% | |||||
14 | Serum Albumin | Serum albumin | ALB | P02768 | 0.5 | 0.000 | 967 | 152 | 32 | 54% | ||
16 | Serum Albumin | Serum albumin | ALB | P02768 | 0.5 | 0.001 | 2741 | 425 | 45 | 71% | ||
17 | Elongation factor 2 | EF2 | EEF2 | P13639 | 0.6 | 0.007 | 675 | 67 | 30 | 36% | ||
18 | Long chain fatty acid-CoA ligase 1 (Acsl1) | Acsl1 | ACSL1 | P33121 | 2.6 | 0.002 | 587 | 46 | 14 | 26% | 9.8 | 0.037 |
19 | Phosphoenolpyruvate carboxykinase [GTP], mitochondrial | PEPCK-M | PCK2 | Q16822 | 0.4 | 0.024 | 142 | 4 | 3 | 5% | ||
20 | Phosphoenolpyruvate carboxykinase [GTP], mitochondrial | PEPCK-M | PCK2 | Q16822 | 0.5 | 0.002 | 1201 | 61 | 15 | 24% | ||
21 | Fascin | Fascin, p55 | FSCN1 | Q16658 | 2.3 | 0.001 | 11324 | 770 | 31 | 62% | 1.3 | 0.027 |
Glutamate dehydrogenase 1, mitochondrial | GDH1 | GLUD1 | P00367 | 417 | 95 | 21 | 41% | 1.4 | 0.006 | |||
22 | Fascin | Fascin, p55 | FSCN1 | Q16658 | 2.1 | 0.001 | 9861 | 593 | 37 | 74% | ||
Glutamate dehydrogenase 1, mitochondrial | GDH1 | GLUD1 | P00367 | 180 | 30 | 15 | 29% | |||||
23 | UTP—glucose-1-phosphate uridylyltransferase | UDPGP | UGP2 | Q16851 | 1.7 | 0.007 | 2302 | 161 | 18 | 38% | 2.2 | 0.000 |
Fascin | Fascin, p55 | FSCN1 | Q16658 | 244 | 38 | 7 | 15% | |||||
24 | Aspartate aminotransferase, cytoplasmatic | cAspAT | GOT1 | P17174 | 0.5 | 0.001 | 10141 | 736 | 41 | 77% | ||
25 | Guanosine monophosphate reductase 2 | GMP reductase | GMPR2 | Q6PKC0 | 0.6 | 0.027 | 750 | 86 | 19 | 53% | ||
26 | Annexin A2 isoform 2 | Annexin A2 | ANXA2 | P07355 | 0.3 | 0.002 | 2994 | 227 | 31 | 77% | ||
27 | Dicarbonyl reductase HEP27 | HEP27 | DHRS2 | Q13268 | 0.5 | 0.002 | 530 | 39 | 20 | 68% | ||
28 | Dicarbonyl reductase HEP27 | HEP27 | DHRS2 | Q13268 | 0.5 | 0.004 | 8124 | 441 | 32 | 85% | ||
29 | Dicarbonyl reductase HEP27 | HEP27 | DHRS2 | Q13268 | 0.4 | 0.001 | 5456 | 304 | 22 | 69% | ||
30 | Dicarbonyl reductase HEP27 | HEP27 | DHRS2 | Q13268 | 0.4 | 0.000 | 10917 | 711 | 47 | 87% |
a adjusted for FDR,
b one tailed Welch t test for unequal variance.