Table 1. Phosphoacceptors identified after purification of M. tuberculosis ParB from the E. coli BL21(DE3)star strain co-expressing M. tuberculosis PknB.
| Phosphorylated tryptic peptide sequence of ParB purified from pCDFDuet co-expressing PknB | Number of detected phosphate groups (LC/MS/MS) | Phosphorylated residue(s) |
|---|---|---|
| [15–28] ENLYFQGHMTQPpSR | 1 | S5 |
| [36–58] GLAALIPTGPADGESGPPpTLGPR | 1 | T32 |
| [42–58] PTGPADGESGPPpTLGPR | 1 | T32 |
| [59–83] MoxGSApTADVVIGGPVPDTSVM GAIYR | 1 | T41 |
| [59–83] MGSApTADVVIGGPVPDpTSVM GAIYR | 1 | T41+T53 |
| [59–83] MGSATADVVIGGPVPDpTSVMGAIYR | 1 | T53 |
| [126–143] SLAGSQpTGVRYQIVMGER | 1 | T110 |
| [211–220] SRPLIpTNMIR | 1 | T195 |
| [244–261] ALLSLEAGPEAQEELApSR | 1 | S239 |
| [244–270] ALLSLEAGPEAQEELApSRIVAEGLSVR | 1 | S239 |
| [271–285] ATEEpTVTLANHEANR | 1 | T254 |
| [271–299] ATEEpTVTLANHEANRQAHHSDATTPAPPR | 1 | T254 |
| [315–329] LSTTFDpTRVTVSLGK | 1 | T300 |
| [315–330] LSTTFDpTRVTVSLGKR | 1 | T300 |
| [315–330] LSTTFDpTRVpTVSLGKR | 1 | T300+T303 |
| [315–329] LSTTFDTRVpTVSLGK | 1 | T303 |
| [315–330] LSTTFDTRVpTVSLGK | 1 | T303 |
Sequences of the phosphorylated peptides identified in ParB as determined by mass spectrometry following tryptic digestion are indicated, and phosphorylated residues (pT or pS) are shown in bold.