Fig 4. Lower Limit of Detection Dependence on Total Interrogated Volume.

Total interrogated volume influences the lower limit of detection for a given number of partitions. In the plot, precision curves for a given number of partitions (20,000) are shown where the difference is in the size of the partitions (1, 2, 10, 20, and 40 nanoliters). The larger the total interrogated volume increases (due to increase in partition size), the smaller the lower limit of detection. The colored dots represent the lower limit of detection for the various partition sizes at 10% precision.