| 1. Review the NTC (non-template control) chips to ensure that false positive rate for your assay system is acceptable (Typically <50 positives). |
Three NTC were run with 4, 13 and 35 positives and thus this criteria was passed. |
| 2. For any chip that is not a green flag, review and manually threshold if needed, rejecting chips if you are not able to see a clear manual threshold. |
Manual Thresholds applied (supplemental data provide details for each chip). No chips were rejected. |
| 3. For a given sample, if at least one of the chips is within the preferred concentration range (200–2000 copies/μl), filter out the chips outside of the range. |
Rule applied rejecting data points as shown in Fig. 15. The dilute points for samples D and E were not run as we expected results under 200 copies/μl and they would be rejected by Rule 3. |
| 4. If both chips are within the preferred concentration range (200–2000 copies/μl), and are not within 30% of the expected 50X fold dilution discard results. |
Rule applied with no chips discarded |
| 5. If all chips are above 4600 copies/ul, use the lowest concentration. |
Rule not applied as no paired set of chips were both above 4600 copies/μl |
| 6. If all chips are below 9 copies/μl, use the highest concentration |
Rule not applied as no paired set of chips were both under 9 copies/μl |
