Table 1.
Effect of chronic caffeine ingestion on receptors and ion channels in brain membrance from male NIH Swiss Strain mice.
| Receptor (Ligand) | Bmax (fmol/mg protein) | |
|---|---|---|
| Control | Chronic Caffeine |
|
| A1-Adenosine | ||
| (CHA) | 911 ± 23 | 1089 ± 39** |
| (CHA, striatum) | 688 ± 14 | 767 ± 28** |
| A2A-Adenosine | ||
| (CGS 21680, striatum) | 872 ±57 | 884 ± 44 |
| α1-Adrenergic | ||
| (Cionidinc) | 175 ± 7 | 189 ± 12 |
| α2-Adrenergic | ||
| (Prazosin) | 200± 3 | 193 ± 2 |
| β1-Adrenergic | ||
| (DHA) | 224± 9 | 167 ± 5* |
| β1-Adrenergic | ||
| (DHA, cerebellum) | 158 ± 12 | 115 ± 11* |
| D1-Dopamine | ||
| (SCH 23390, striatum) | 3097 ± 81 | 3165 ± 66 |
| D2-Dopamine | ||
| (Spipcrone, striatum)‡ | 729 ± 21 | 725 ±55 |
| 5-HT1 | ||
| (Serotonin) | 361 ± 14 | 474 ± 48** |
| 5-HT2 | ||
| (Ketanserin) | 275 ± 11 | 347 ±11* |
| Nicotinic | ||
| (Nicotine) | 34 ± 2 | 50 ± 3* |
| (Nicotine, striatum) | 37 ± 1 | 39 ± 2 |
| Muscarinic | ||
| (Quinuclidinyl benzilate) | 1153 ±56 | 1509 ± 47** |
| NMDA | ||
| (MK-801) | 2653 ± 97 | 2588 ± 46 |
| GABAA | ||
| (Diazepam) | 1061 ± 69 | 1741 ± 100* |
| Opioid | ||
| mu (DAMGO) | 119 ± 6 | 134 ± 5 |
| delta (DPDPE) | 83 ±2 | 104 ± 7** |
| kappa (U69593) | 339 ± 65 | 230± 48 |
| Sigma | ||
| (DTG) | 2580± 90 | 2560± 170 |
| Ca2+ Channel | ||
| (Nitredinine} | 314 ± 6 | 369 ± 12** |
Binding of radioligands to conical membranccs or as noted to cerebellar or striatal mcmbr.ances from control mice and chronic caffeine mice. Values for Bmax are means ± S.E.M. (Shi et al., 1993, 1994).
*
p<0.01
**
p<0.05.
‡
no significant change when assayed with [3H] raclopride.