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. 2015 Mar 25;11(3):e1005106. doi: 10.1371/journal.pgen.1005106

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© 2015 Encinar del Dedo et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 6 — (A) Upon long DIP treatments, Grx4 and Fra2 are dispensable for activation of Fep1-dependent genes. Total RNA from YE cultures of strains 972 (WT), NG81 (Δgrx4) and NG101 (Δfra2), before and after the indicated time in hours with DIP, was processed as described in Fig. 1E. (B) Scheme of the metal transfer reaction between GST beads-bound GST-Fep1^1–245 and Grx4-apo-Fra2. (C and D) In vitro Fe transfer from Fe-Fep1 to apo-Grx4-Fra2. UV/visible spectra of GST-tagged Fep1 (C) and Grx4-Fra2 (D) were recorded before (dashed line) and after (solid line) incubation in a 1:1 protein ratio and protein separation through GSH-affinity chromatography. (E) Model proposed for the reverse metal transfer reaction between Fe (solid circles)-containing Fep1 and Fe-depleted Grx4-Fra2. See text for details.