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. 2015 Mar 25;10(3):e0120526. doi: 10.1371/journal.pone.0120526

Fig 2. Time course of the morphological effects of PACAP and BDNF on cultured hippocampal neurons during 3 DIV.

Fig 2

Primary hippocampal neurons cultured with 10 nM PACAP or 2 nM BDNF were immunostained for MAP2 on DIV 1, 2, and 3. (A-D) Time-dependent effects of PACAP (closed circles), BDNF (closed triangles), and vehicle (open circles) on total neurite length (A), number of total (B) and primary (C) neurites, and soma size (D). Values represent mean ± SEM of 60–75 neurons from three independent experiments. *P < 0.05, **P < 0.01 vs. control at the same DIV, ## P < 0.01 vs. BDNF at the same DIV, two-way ANOVA followed by Tukey-Kramer test.