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. 2015 Mar 25;10(3):e0120526. doi: 10.1371/journal.pone.0120526

Fig 3. PACAP and BDNF comparably increase axon, but not dendrite, length.

Fig 3

Primary hippocampal neurons were cultured with 10 nM PACAP or 2 nM BDNF for 1 to 3 DIV and double-immunostained for pNF and MAP2. (A) Representative pNF- (red) and MAP2- (green) immunostained images of neurons. (B, C) Time-dependent effects of PACAP (closed circles), BDNF (closed triangles), and vehicle (open circles) on axon (B) and dendrite (C) length. Values represent mean ± SEM of 60 neurons from three independent experiments. **P < 0.01, two-way ANOVA followed by Tukey-Kramer test. Scale bar, 20 μm.