Fig 5. The effect of TrkB receptor inhibitor K252a on neurite outgrowth.

Primary hippocampal neurons were cultured with 10 nM PACAP or 2 nM BDNF in the presence or absence of 200 nM K252a for 3 DIV and double-immunostained for pNF and MAP2. Representative pNF- (red) and MAP2- (green) immunostained images of neurons (A), axon length (B), and dendrite length (C) were shown. Values represent mean ± SEM of 60 neurons from three independent experiments. **P < 0.01 vs. control, ^## P < 0.01 vs. without K252a, two-way ANOVA followed by Tukey-Kramer test. Scale bar, 20 μm.