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. 2015 Mar 25;10(3):e0119136. doi: 10.1371/journal.pone.0119136

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© 2015 Hardy et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 2 — A. Field of view of MIN6 cells transfected with the pCMV6-Zip4GFP plasmid. B. Expression of Zip4 was measured by qPCR performed on MIN6 cells transfected with pCMV6-Zip4GFP plasmid C. Co-localization was estimated with the Pearson correlation coefficient analysis calculated from confocal images obtained from the FITC and Cy3 channel. D. Confocal images of pCMV6-Zip4GFP transfected MIN6 cells after staining of the nucleus with TOPRO-3, plasma membranes with WGA Alexa Fluor 555, mitochondria with MitoTracker Red CMXRos or the endoplasmic reticulum (ER) with immunostaining of KDEL.