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. 2015 Mar 25;10(3):e0119136. doi: 10.1371/journal.pone.0119136

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© 2015 Hardy et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — A. Fluorescent images (left panels) and 2 representative traces (right panel) of zinc cytoplasmic levels measured with FluoZin-3AM in MIN6 control and pCMV6-Zip4GFP transfected MIN6 cells. B. Following supplementation of 100 μM Zinc, the rate of zinc increase was calculated by applying a linear curve fit and calculating its slope over the first 400 seconds of the fluorescence increase. C. The overall area under the FluoZin-3AM curve in response to 100 μM Zn²⁺ was calculated in control MIN6 and pCMV6-Zip4GFP transfected MIN6 cells. D. Granular zinc content was estimated with the low affinity zinc dye zinquin in MIN6 cells transfected with pcDNA-eGFP (control condition) and pCMV6-Zip4GFP. E. Overall zinquin intensity in control MIN6 and pCMV6-Zip4GFP transfected MIN6 cells. *. p˂0.05; ***. p˂0.001.