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. 2015 Mar 25;10(3):e0122176. doi: 10.1371/journal.pone.0122176

Fig 2. The identity of the intercodon influences −1 frameshift efficiency.

Fig 2

Top: schematic representations of the base of the HIV-1 stem-loop, with the natural glycine codon and variants (left) and the substituted stop codon and variants showing the intercodon (grey) and any complementary stem-loop alterations (bold). The end of the slippery sequence is underlined. Below: frameshift efficiency of the natural intercodon and variants as assayed with the dual luciferase reporter system. GGG_AA and UGA_U indicate the intercodon (left part) and the modification to the complementary sequences in the stem-loop (right part). The mean ± standard error of the mean (SEM) for 18 replicates from three individual experiments in COS-7 cells is shown. ***P = < 0.001 compared to the GGG intercodon.