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. 2015 Mar 25;10(3):e0122149. doi: 10.1371/journal.pone.0122149

Fig 4. IGF-1R, c-Met, and EGFR but not EpoR are functional in primary tumor cell populations from human tumor tissues.

Fig 4

Disaggregated tumors were stimulated with a range of concentrations of rHuEpo and growth factor controls for 5 and 30 minutes. These time points were chosen based upon experiments in the UT-7/Epo cell-line showing that phosphorylation peaked between 15 and 30 minutes for the majority of signaling proteins assayed (S7 Fig.). In disaggregated tumors, identical data were obtained for 5 and 30 minutes; therefore only the 5-minute data are shown. On each plot, the dotted line represents the threshold for pAKT (1.14) and pERK (1.16). Data shown for (A) cell-line controls (i) UT-7/Epo (ii) HT29 (n = 38 each cell-line) and tumors from (B) colorectal (n = 45), (C) breast (n = 34), (D) non-small cell lung (n = 43), (E) ovarian (n = 31). Levels are reported as a ratio of mean fluorescent intensity (MFI) values relative to the appropriate isotype control. (Note: the ratio to isotype was used to normalize data and does not serve as baseline measure; i.e., a ratio of 1 does not imply a lack of receptor expression).