Fig 2. CD4 binding to the BG505 SOSIP.664 trimer and the induction of CD4-like conformational changes.

(A) Use of sCD4 as a competitor. (B) Use of CD4-IgG2 as the analyte. (C) Induction of conformational changes measured by 17b binding. The extent of 17b binding in the presence of sCD4 was defined as 100%. *** indicates a significant difference between 17b binding without sCD4 and in the presence of a bNAb, as calculated using a Mann-Whitney 2-tailed test (P <0.05).