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. 2015 Mar 25;10(3):e0121320. doi: 10.1371/journal.pone.0121320

Fig 3. OCH administration increased cell infiltrates and activation of T cells in mice.

Fig 3

(A) C57BL/6 mice were intravenously injected with α-GalCer, OCH, or PBS. Liver total mononuclear cells (MNC) were counted 3 days after α-GalCer, OCH, or PBS injection. n = 10–13 mice per group. ***, p<0.001. (B-E) Wild type mice were immunized with 2-OA-BSA and α-GalCer (group name: 2-OA/a-GC), OCH (group name:2-OA/OCH) or PBS (group name: 2-OA/PBS) at weeks 0, 2, 4, 6 and 8 and sacrificed at week 12. (B) Liver total mononuclear cells (MNC) were measured. (C) The numbers of T (CD3+ NK1.1-), NKT (CD3+NK1.1+), NK (CD3-NK1.1+) and B (CD19+) cells were measured. (D) The numbers of CD4+ and CD8+ T cells were detected. (E) The expression of CD69 and CD44 in CD4+ and CD8+ T cells was measured by flowcytometry. n = 9–10 mice per group. *, p<0.05; **, p<0.01; ***, p<0.001.