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. Author manuscript; available in PMC: 2016 Apr 2.
Published in final edited form as: FEBS Lett. 2015 Mar 3;589(8):885–889. doi: 10.1016/j.febslet.2015.02.030

Figure 1.

Figure 1

(A, left) Equilibrium distribution of a donor fluorophore (red dot) on the outer membrane leaflet and dipicrylamine (DPA –purple dot) in the absence of an external field, E=0. (right) After the application of a pulsed electric field, E=Ej, DPA preferentially migrates to a leaflet. (B) Change in the distance between the fluorophone (red objects) and DPA (blue objects) in various locations as indicated in panel A before (a-c) and after (d-f) the application of the pulsed electric field. (C) The fluorescence intensity (F.I.) of the fluorophore will be affected by changes in the DPA location due to FRET. When its distance to the DPA is closer (as in d), the intensity goes down. An increase in its intensity, however can be observed, as DPA moves further (as in f) from its equilibrium position. Dotted lines show changes in intensity if the fluorophore was on the opposite leaflet.