Figure 4.
(A) Fluorescence image of PC12 cell labeled with 1 µM of FM1-43. Data are collected at positions marked (+) and (×) on the cell membrane. Scale bar = 10 µm. The polarity of the electric field, E, is indicated by the “+” and “−“ signs. (B) Structure of FM1-43. (C) Optical response in the absence of DPA. (D) Optical response in the presence of 10 µM DPA. (E) Averaged signal for experimental conditions as in [D], n=3. E=166 V/cm, pulse width = 20 ms in traces [C]-[E].