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IFN-gamma producing splenocytes in ELISPOT assay after treatment with various conditions. Mice were injected subcutaneously on both flanks on day 0 with a total dose of 2×1011 AuNP-OVA and 1012 AuNP-CpG, or the equivalent of at most 50 μg OVA and approximately 4.7 μg CpG. The dose was repeated on day 10, and the spleens were harvested on day 17. *, p<0.02.
