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. 2015 Feb 12;106(3):227–236. doi: 10.1111/cas.12593

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© 2014 The Authors. Cancer Science published by Wiley Publishing Asia Pty Ltd on behalf of Japanese Cancer Association.

This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Functional domains of PU.1 (a) Deletion mutants of PU.1. The MOZ- and mixed lineage leukemia (MLL)-interacting domains and the results for interaction with PU.1 and PU.1-mediated transactivation of macrophage colony-stimulating factor receptor (Csf1r)-luc are indicated. ND, not determined. (b) Interaction of PU.1 mutants with MLL. 293T cells were co-transfected with HA-tagged MLL and WT or mutants of FLAG-tagged PU.1. Anti-FLAG antibody immunoprecipitates (IP:FLAG) or cell lysates (Input) were subjected to immunoblotting with anti-HA or anti-FLAG antibodies. (c) SaOS2 cells were co-transfected with the Csf1r–luciferase construct and the indicated effectors. Luciferase activity was analyzed 24 h after transfection. Error bars represent SD (n = 3).