Table 1.
Comparison of the binding of WW1 domain to various PPXY-containing peptides derived from putative ligands of WWOX alone (WW1) and in the context of WW1–WW2 tandem module (WW1–WW2) in terms of the apparent equilibrium dissociation constant (Kd). (A color version of this table is available in the online journal.)
| Ligand | Peptide Sequence | Kd/µM |
|
|---|---|---|---|
| WW1 | WW1–WW2 | ||
| ErbB4 | TVLPPPPYRHRN | 179 ± 10 | 75 ± 8 |
| p73 | HCTPPPPPYHADP | 107 ± 9 | 60 ± 6 |
| PTCH1 | RYSPPPPYSSHS | 278 ± 19 | 79 ± 8 |
| PTPN14 | LFRPPPPYPRPR | 763 ± 62 | 214 ± 30 |
| SMAD7 | LESPPPPYSRYP | 272 ± 29 | 53 ± 8 |
| TMG2 | HDAPPPPYTSLR | 202 ± 23 | 84 ± 9 |
| WBP1 | PGTPPPPYTVAP | 368 ± 44 | 141 ± 12 |
| WBP2 | SQPPPPPYYPPE | 133 ± 18 | 42 ± 7 |
Note that the consensus residues within the PPXY motif of each peptide are colored red for clarity. All parameters were obtained from isothermal titration calorimetry (ITC) measurements conducted at 25℃ and pH 7. Errors were calculated from at least three independent measurements to one standard deviation. (A color version of this table is available in the online journal).