Figure 3.

Effect of LPS and IL-1β and matrix metalloproteinase inhibitor, GM6001, on syndecan-4 shedding. (A) HUVECs were exposed to LPS or IL-1β for 4 and 24 h. Supernatants were harvested and the amount of syndecan-4 in the conditioned media was quantified by ELISA (n=6). (B) Syndecan-4 levels in the conditioned media and in cell lysates were determined by ELISA after HUVECs received a 24-h incubation with 5 or 25 µM GM6001 (n=4). A value of p<0.05 is considered a statistically significant difference between the treatment and control. *p<0.05, ***p<0.001.