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. Author manuscript; available in PMC: 2015 Sep 15.
Published in final edited form as: J Immunol. 2015 Feb 11;194(6):2654–2663. doi: 10.4049/jimmunol.1401288

Figure 6. Reduced proliferation and Th1 and Th17 responses in BDC-Idd9.905 CD4+ T cells following stimulation with BDC2.5 mimotope.

Figure 6

Purified CD4+ T cells from BDC-Idd9.905 or BDC mice were stimulated with indicated concentrations of BDC2.5 mimotope p79 in presence of irradiated NOD spleen cells as APCs for 3 days. (A) CD4+ T cell proliferation was determined by [3H]thymidine incorporation assay and shown as mean counts per minute (CPM) of triplicate cultures. (B) Concentrations of indicated Th cytokines in supernatants of p79-stimulated CD4+ T cell cultures were assayed in duplicate by ELISA. One of three independent experiments each with similar data is shown. Error bars represent SD. * p < 0.03 (A) * p < 0.02 (B) (Student’s t test).