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. Author manuscript; available in PMC: 2015 Aug 18.
Published in final edited form as: Nat Commun. 2015 Feb 18;6:6243. doi: 10.1038/ncomms7243

Figure 5. N. vectensis Grl1 is required for arboral pole patterning.

Figure 5

(a) Morphological phenotypes of N. vectensis embryos injected with either control (control#1) or NvecGrl1 (NvecGrl1#1) morpholino oligonucleotides, in which DNA (magenta) and the actin cytoskeleton (green) are labelled with TO-PRO-3 and Alexa Fluor 488 Phalloidin, respectively. The arrow marks the group of nuclei at the aboral pole that have translocated to a more basal position (towards the right of the image) in control but not NvecGrl1 morphants; this results in these cells forming a small indentation of the apical ectoderm (arrowhead). Scale bar = 100 μm (applies to all images). Quantification of the phenotypes is shown below.

(b) Bright-field images of living 4 day planulae derived from N. vectensis embryos injected with either control (control#1) or NvecGrl1 (NvecGrl1#2) morpholino oligonucleotides revealing the failure in apical tuft develops in NvecGrl1 morphants. Quantification of the phenotypes is shown below. Scale bars = 100 μm.

(c) RNA in situ hybridisation using probes against marker genes in animals injected with either control (control#1) or NvecGrl1 (NvecGrl1#1) morpholino oligonucleotides. Scale bar = 100 μm (applies to all images). Quantification of the phenotypes is shown below.

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