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. 2015 Feb 3;14:24. doi: 10.1186/s12943-014-0284-y

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© Koumangoye et al.; licensee BioMed Central. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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miR-31 is downregulated in invasive esophageal cancer cells. (A, B) Morphologic, migration and invasive capability of two ESCC cells lines and two EAC cell lines were analyzed by bright field microscopy and Boyden chamber transwell assays. (C) Fold change in expression of 18 miRNAs between the invasive EAC cell line FLO1 and non-invasive cell line OE33. (D-F) Quantitative RT-PCR for relative expression of miR-31. miR-31 expression was normalized to RNU6. (D) High expression of miR-31 in non-invasive ESCC TE11 cells versus invasive TE8 cells. (E) Higher expression in the CP-A cell line compared to CP-B and (F) non-invasive EAC OE33 compared with FLO1. Means ± SD from at least three biological replicates.