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. 2015 Mar 26;6:187. doi: 10.3389/fpls.2015.00187

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Copyright © 2015 Chettoor and Evans.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Maize ovules with mature embryo sacs. (A) Fixed and cleared ovule stained with Acriflavine (Red) and Propidium Iodide (Green). Inset shows higher magnification of antipodal cell region. (B) Live cell imaging of an ovule expressing pHistoneH1B::HISTONEH1B-YFP. (C,D) Fixed and cleared ovules stained with Acriflavine (Red) and fluorescence from formaldehyde fixation and autofluorescence (Green). a, antipodal cells; cc, central cell; e, egg cell; n, nucellus; pn, polar nuclei; s, synergid. Scale bar = 100 μm.