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. Author manuscript; available in PMC: 2015 Mar 26.
Published in final edited form as: Apoptosis. 2008 Jun;13(6):790–802. doi: 10.1007/s10495-008-0212-y

Fig. 5.

Fig. 5

Combined treatment of melanoma lines with γ-irradiation and TRAIL. (a) Effect of γ-irradiation on TRAIL-R2/DR5 surface expression. Immunostaining with anti-DR5-PE and FACS analysis were used. (b). Upregulation of TRAIL-induced apoptosis in WM35 melanoma cells after sequential treatment with γ-irradiation (5 Gy) and 16 h after irradiation with recombinant TRAIL (50 ng/ml) for an additional 24 h. Cell cycle-apoptosis analysis was performed using PI staining DNA and flow cytometry. (c) Clonogenic survival assay of WM35, WM9 and LU1205 cells 12 days after treatment with recombinant TRAIL (50 ng/ml) alone or in combination with γ-irradiation of 1.25–5 Gy. Error bars represent mean ± SD from three independent experiments