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. Author manuscript; available in PMC: 2015 Mar 26.
Published in final edited form as: Oncogene. 2010 May 17;29(27):3990–3996. doi: 10.1038/onc.2010.137

Figure 2.

Figure 2

p53 deficiency enhances viral replication and virusinduced apoptosis. (a) MEF p53+/+ and −/− cells were infected with reovirus at multiplicity of infection (MOI) of 40 or myxoma virus at MOI of 5. At 3 days after infection, cells and supernatant are harvested and frozen and thawed three times. Viral titer was determined by plaque assays using L929 cells (reovirus) or RK13 cells (myxoma) and expressed as PFU per cell. (b) MEF p53+/+ and −/− cells were either mock infected or exposed to reovirus for 9 days, then stained for DNA content with DAPI and for apoptotic activity with 7AAP. MEF p53−/− cells showed extensive cellular fragmentation and apoptosis in response to reovirus, whereas MEF p53+/+ cells did not.