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Basolateral ATP causes an intracellular alkalization in perfused medullary thick ascending limb (mTAL). (a) Representative trace of a perfused mTAL loaded with 2′,7′-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethylester (BCECF AM). ATP (100 μm) is applied to the bath for 2 min. (b) Summarized data of the experiments with 100 μm basolateral ATP, n = 14. *Indicates statistical significance (P < 0.001).
