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. 2015 Mar 26;9:107. doi: 10.3389/fncel.2015.00107

Figure 3.

Figure 3

GluRIII glutamate receptor subunits are dramatically decreased in the T15 line. (A-L) Immunostaining of wild type (WT), GAL4 Cont × WT, and T15 × WT NMJs with antibodies against GluRIII (red), Bruchpilot (Brp, green), and HRP (blue). A, E, and I show 40X images (scale bars, 10 μm) of muscle 6/7 NMJs from wandering third instar larvae. (B–D,F–H,J–L) Panels show various channels of 60X images (scale bars, 5 μm) of NMJs. (M) Quantification of the number of presynaptic active zones (marked by Brp) and GluRIII clusters at the muscle 6/7 synapse of segments A2 and A3 (*p < 0.05). (N) Calculation of total GluRIII levels per unit of synapse area. This measure takes into account both GluRIII cluster size and GluRIII intensity (see text for individual values; see Materials and Methods for details; *p < 0.05 compared to WT; ***p < 0.001). (O) Quantification of the number of boutons at segment A2 and A3 muscle 6/7 NMJs. n ≥ 6 NMJs stained for each condition.