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Activation of the human P2Y2 (left panel), and P2Y4 (right panel) receptors by chiral α-phosphothioate uracil nucleotide analogues 21 (▲), 22 (◆), and 23 (▼). PLC activity was measured as described in Section 3.2 in 1321N1 human astrocytoma cells stably expressing the human P2Y receptors. The data are the means of triplicate determinations and are representative of results obtained in three separate experiments.
