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. Author manuscript; available in PMC: 2015 Mar 26.
Published in final edited form as: J Biol Chem. 2003 Oct 13;279(2):1304–1309. doi: 10.1074/jbc.M310389200

Fig. 4. PI3-kinase signaling is involved in adiponectin-induced angiogenic pathway.

Fig. 4

A, quantitative analysis of tube formation is shown. HUVECs were treated with adiponectin (30 µg/ml) or BSA (30 µg/ml) in the presence of LY294002 (10 µm) or vehicle at the time of seeding. B, a modified Boyden chamber assay was performed using adiponectin as the chemoattractant. HUVECs were pretreated with LY294002 (10 µm) or vehicle for 1 h and then incubated with adiponectin (30 µg/ml) or BSA (30 µg/ml) for 4 h. C, effects of LY294002 on adiponectin-stimulated protein phosphorylation (p-). Representative blots are shown. HUVECs were pretreated with LY294002 (10 µm) or vehicle for 1 h and then incubated with adiponectin (30 µg/ml) or BSA (30 µg/ml) for the indicated lengths of time. Results are presented as the mean ± S.E. For A and B, results are expressed relative to control. *, p < 0.01.