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. Author manuscript; available in PMC: 2015 Mar 26.
Published in final edited form as: J Biol Chem. 2003 Oct 13;279(2):1304–1309. doi: 10.1074/jbc.M310389200

Fig. 5. Adiponectin promotes angiogenesis in vivo.

Fig. 5

An in vivo Matrigel plug assay was performed to evaluate the effect of adiponectin on angiogenesis (A and B). Matrigel plugs containing adiponectin (100 µg/ml, n = 3) or phosphate-buffered saline (Control, n = 3) were injected subcutaneously into mice. A, plugs were stained with the endothelial cell marker CD31. Bar, 100 µm. B, the frequency of CD31-positive cells in five low power fields was determined for each Matrigel plug. Data were presented as fold increase of CD31-positive cells relative to the control. A rabbit cornea assay was performed (C and D). Pellets containing adiponectin (1 and 10 µg, n = 8), VEGF (100 ng, n = 8), or phosphate-buffered saline (Control, n = 8) were implanted in the cornea. C, photographs of rabbit eyes are shown (Control, adiponectin 10 µg; VEGF, 100 ng). D, an angiogenic score was calculated (vessel density × distance from limbus). Results are shown as the mean ± S.E. *, p < 0.01 versus control.