Figure 5. Localization of RBG-1 on synaptic vesicles requires SNT-1.
(A and B) mCherry::RBG-1 has a punctate distribution in wild type (A) but accumulates in cell bodies (yellow arrows) in unc-104 mutants (B). (C) mCherry::RBG-1 (red) is co-localized with SNB-1::GFP puncta (green). (D) In snt-1 mutants, mCherry::RBG-1 loses its punctate localization and becomes diffuse in axons, while SNB-1::GFP retains its punctate pattern. (E) mCherry::RBG-1 retains its punctate distribution and is co-localized with SNB-1::GFP in rab-3 mutants. Scale bars, 5 µm.