Table 1.
Quantitative summary of cellular and synaptic properties of Disc1 and control PrlC neurons
| Parameter | Disc1 | Control | P/N | Test | Cohen's d | |
|---|---|---|---|---|---|---|
| Sucrose preference | 1.46 ± 0.07 | 1.44 ± 0.15 | 0.901, N = 10/10 | Student's t-test | na | |
| Freezing in TST (norm.) | 1.39 ± 0.12 | 1.00 ± 0.10 | 0.015, N = 22/14 | Student's t-test | 0.82 | |
| Immobility in forced swim test (norm.) | 1.18 ± 0.07 | 1.00 ± 0.05 | 0.049, N = 22/20 | Student's t-test | 0.65 | |
| Open field total path length (m) | 14.5 ± 0.9 | 19.3 ± 0.7 | 0.0012, N = 19/18 | Student's t-test | 1.47 | |
| Open field path length (first half/second half) | 4.8 ± 0.4/8.9 ± 0.5 | 9.8 ± 0.5/9.1 ± 0.3 | 1.1*10−12, 0.682 for s half | One-way ANOVA followed by t-test | na (second half) | |
| Radial arm maze reference/working memory errors | 22 ± 5/14 ± 2 | 25 ± 2/17 ± 3 | 0.239/0.361, N = 6/9 | Mann–Whitney U test | na | |
| Proportion spontaneous alternation | 0.60 ± 0.03 | 0.65 ± 0.04 | 0.361, N = 8/8 | Student's t-test | na | |
| Extradim. spatial/shifted rule learning trial (y-maze) | 10 ± 1/39 ± 7 | 15 ± 3/34 ± 9 | 0.154/0.632, N = 8/7, 6/5 | Student's t-test | na | |
| Time in center of open field (%) | 43.5 ± 0.9 | 37.4 ± 3.5 | 0.377, N = 19/18 | Student's t-test | na | |
| 3-chamber social interaction: stranger preference | 49.4 ± 4.6 | 53.2 ± 3.3 | 0.513, N = 9/9 | Student's t-test | na | |
| TST-dependent cFos increase PrLC (norm.) | 3.92 ± 1.54 | 4.78 ± 1.01 | 0.03/0.04, N = 4/3 | Mann–Whitney U test | 1.52/3.49 | |
| Freezing in TST (%, electrode-implanted sample) | 52.2 ± 0.1 | 29.4 ± 0.1 | 0.004, N = 8/6 | Mann–Whitney U test | 1.82 | |
| TST: low-gamma power/amplitude (*10−3) | 0.11 ± 0.02/0.87 ± 0.05 | 0.29 ± 0.04/1.34 ± 0.09 | 0.003/0.002, N = 8/6 | Mann–Whitney U test | 2.33/2.77 | |
| TST: theta power/amplitude (*10−3) | 0.11 ± 0.03/0.04 ± 0.004 | 0.29 ± 0.04/0.07 ± 0.004 | 0.012/0.001, N = 8/6 | Mann–Whitney U test | 1.97/3.12 | |
| Home cage: low-gamma power (*10−3) | 0.12 ± 0.02 | 0.22 ± 0.03 | 0.009, N = 8/6 | Mann–Whitney U test | 1.62 | |
| Home cage: theta power (*10−3) | 0.1 ± 0.02 | 0.18 ± 0.04 | 0.031, N = 8/6 | Mann–Whitney U test | 1.29 | |
| Urethane anesthesia: low-gamma power (*10−3) | 0.6 ± 0.2 | 1.1 ± 0.2 | 0.024, N = 11/7 | Student's t-test | 1.27 | |
| TST: hippocampal theta power (*10−3) | 0.8 ± 0.2 | 4.1 ± 1.5 | 0.009, N = 5/4 | Mann–Whitney U test | 1.71 | |
| CA1-PrLC theta coherence TST/home cage | 0.52 ± 0.06/0.52 ± 0.07 | 0.52 ± 0.04/0.49 ± 0.02 | 0.972, N = 4/3 | One-way ANOVA | na | |
| PV-IN count PrLC all layers (normalized) | 0.69 ± 0.08 | 1.00 ± 0.04 | 0.004, N = 9/8 | Student's t-test | 1.81 | |
| PV-IN count layer 2–3/layer 5 | 3.0 ± 0.7/31.9 ± 5.3 | 6.2 ± 0.54/48.8 ± 2.52 | 0.007/0.011, N = 6/5 | Mann–Whitney U test | 2.35/1.86 | |
| Somatostatin-IN count (normalized) | 1.04 ± 0.10 | 1.00 ± 0.08 | 0.392, N = 6/5 | Mann–Whitney U test | na | |
| DAPI density PrLC (normalized) | 0.93 ± 0.04 | 1.00 ± 0.04 | 0.158, N = 6/5 | Mann–Whitney U test | na | |
| Calbindin-IN count (normalized) | 0.75 ± 0.12 | 1.00 ± 0.05 | 0.027, N = 6/5 | Mann–Whitney U test | 1.2 | |
| PV-VGAT-positive boutons PrLC (normalized) | 0.62 ± 0.09 | 1.00 ± 0.10 | 0.021, N = 6/4 | Mann–Whitney U test | 2.02 | |
| FS-IN bouton density in vitro (µm−1) | 0.086 ± 0.011 | 0.073 ± 0.009 | 0.388, N = 12/10* | Student's t-test | na | |
| FS-IN bouton density in vivo (µm−1) | 0.091 ± 0.010 | 0.093 ± 0.010 | 0.868, N = 16/20* | Student's t-test | na | |
| FS-IN axon length in vitro (mm) | 1.54 ± 0.14 | 2.02 ± 0.27 | 0.200, N = 4/4* | Mann–Whitney U test | na | |
| Amplitude (pA) | 50.8 ± 13.3 | 121.1 ± 32.1 | 0.011, N = 20/13* | Mann–Whitney U test | 0.79 | |
| rise time (ms) | 0.32 ± 0.02 | 0.32 ± 0.03 | 0.985, N = 19/13* | Mann–Whitney U test | na | |
| decay time constant (ms) | 5.92 ± 0.40 | 5.28 ± 0.28 | 0.193, N = 10/11* | Mann–Whitney U test | na | |
| onset latency (ms) | 1.01 ± 0.03 | 1.02 ± 0.05 | 0.378, N = 19/13* | Mann–Whitney U test | na | |
| FS-IN-to-PC uIPSC | failure rate | 0.32 ± 0.07 | 0.08 ± 0.04 | 0.015, N = 20/13* | Mann–Whitney U test | 1.06 |
| coefficient of variation | 0.924 ± 0.165 | 0.426 ± 0.072 | 0.008, N = 17/13* | Mann–Whitney U test | 0.92 | |
| skewness | −0.262 ± 0.135 | −0.124 ± 0.174 | 0.503, N = 17/13* | Mann–Whitney U test | na | |
| paired-pulse ratio 20/50 ms | 0.84 ± 0.08/0.85 ± 0.08 | 0.89 ± 0.09/0.82 ± 0.03 | 0.836/0.937, N = 7/6, 6/6 | Mann–Whitney U test | na | |
| multiple-pulse 50 Hz 10th | 0.58 ± 0.06 | 0.54 ± 0.05 | 0.671, N = 8/7* | Student's t-test | na | |
| Connection probability (%) | 34.6 | 12.8 | 5.3*10–29, N = 78/148* | Chi2 test | na | |
| Nr | 4 ± 2 | 10 ± 2 | 0.036, N = 14/11* | Mann–Whitney U test | 0.87 | |
| Binomial fitting | Qr | 27.6 ± 2.6 | 28.5 ± 3.5 | 0.831, N = 14/11* | Student's t-test | na |
| Pr | 0.45 ± 0.07 | 0.58 ± 0.06 | 0.178, N = 14/11* | Student's t-test | na | |
| amplitude (pA) | 30.7 ± 2.3 | 28.7 ± 3.4 | 0.34, N = 15/9* | Mann–Whitney U test | na | |
| spEPSCs on FS-INs | frequency (Hz) | 6.0 ± 1.0 | 9.5 ± 1.1 | 0.022, N = 15/9* | Student's t-test | 1.1 |
| coefficient of variation | 0.75 ± 0.04 | 0.75 ± 0.05 | 0.596, N = 15/9* | Student's t-test | na | |
| amplitude (pA) | 17.9 ± 1.1 | 18.4 ± 3.0 | 0.202, N = 11/13* | Mann–Whitney U test | na | |
| spEPSC on PCs | ||||||
| frequency (Hz) | 3.1 ± 0.4 | 2.6 ± 0.4 | 0.246, N = 11/13* | Mann–Whitney U test | na | |
| amplitude (pA) | 20.1 ± 1.1 | 21.9 ± 1.9 | 0.388, N = 24/15* | Student's t-test | na | |
| mIPSC on PCs | ||||||
| frequency (Hz) | 0.74 ± 0.14 | 1.18 ± 0.23 | 0.025, N = 24/15* | Mann–Whitney U test | 0.57 |
N indicates number of animals except for: *N indicates number of cells, #N indicates number of axons.