Table 1.
Institute | Materials | Plants | Cryo-storage accessions (No.) | Cryopreservation methods |
---|---|---|---|---|
NCGRP, USA | Orthodox seeds | 10% seeds of accession | over 37,000 | Desiccation, (Engelmann personal communication 2014) |
NBPGR, India | Orthodox seeds | 50 species | 1,200 | |
NAB, RDA, Rep. Korea | Orthodox seeds | Panax ginseng | 400 | |
IRD, France | Non orthodox seeds | Coffea spp. | 500 | |
| ||||
NCGRP, USA | Dormant buds | Malus spp. | 2,200 | Cryopreservation using dormant buds (Slow freezing) |
NIAS, Japan | Dormant buds | Morus spp. | 1,236 | |
NBPGR, India | Dormant buds | Morus spp. | 329 | |
AFOCEL, France | Dormant buds | Ulmus spp. | 440 | |
| ||||
NCGRP/NCGR, USA | In vitro shoot tips | Pyrus spp. | 100 | Slow freezing |
NCGRP/NCG, USA | In vitro shoot tips | Rubus spp. et al. | 57 | Slow freezing |
CIAT, Colombia | In vitro shoot tips | Manihot esculenta | 480 | Droplet vitrification |
INIBAP, Belgium | In vitro shoot tips | Musa spp. | 700 | Droplet vitrification |
NICS, RDA, Rep. Korea | Shoot from cloves | Allium sativum L. | 300 | Droplet vitrification |
IPK, Germany | In vitro shoot tips | Allium sativum L. | 101 | Vitrification |
IPK, Germany | In vitro shoot tips | Mentha L. | 86 | Droplet vitrification |
SARC, Japan | In vitro shoot tips | Wasabia japonica M. | 40 | Vitrification |
NIAS, Japan | In vitro shoot tips | Juncus effusus | 50 | D cryo-plate |
These information obtained in 2nd International Symposium on Plant Cryopreservation (Aug. 2013), Fort Collins, Colorado, USA, except seeds. NCGRP (National Center for Genetic Resources Preservation); NCGR (National Clonal Germplasm Repository); NBPGR (National Bureau of Plant Genetic Resources); NAC, RDA (National Agrobiodiversity Center, Rural Development Administration); IRD (Institute de Recherche pour le Développement); NCGR (National Clonal Germplasm Repository); NIAS (National Institute of Agrobiological Sciences); AFOCEL (Association Forêt Cellulose); CIAT (International Center for Tropical Agriculture); INIBAP (International Network for the Improvement of Banana and Plantain); NICS RDA (National Institute of Crop Science, Rural Development Administration); IPK (Leibniz Institute of Plant Genetics and Crop Plant Research); SARC (Shimane Agriculture Research Center).