Table 5.
Validation history of the selection markers for marker-assisted selection on Ryadg that confers extreme resistance to potato virus Y (PVY) located near the proximal end of chromosome XI. Genotype implies representation of cultivars, breeding lines, and clones of wild species from different origins
Marker type | Correspondence with the target Ryadg phenotype | References |
---|---|---|
RFLP (GP 125) | 30/31 genotypes (96.7%) | Hämäläinen et al. (1997) |
RFLP (ADG2 probe, 3.5 kb fragment) | 77/77 F1 progeny 112/117 genotypes (95.7%) |
Hämäläinen et al. (1998) Shiranita et al. (1999) |
CAPS (ADG2 fragment) | 77/77 genotypes (100%) | Sorri et al. (1999) |
RFLP (ADG2 probe, 10.5 kb fragment) | 97/117 genotypes (83%) for different origins of Ry sources | Shiranita et al. (1999) |
SCAR (ADG2 fragment with RYSC3 primers) | 103/103 genotypes (100%) | Kasai et al. (2000) |