Figure 1.
AFM schematics for force mapping of human meniscal tissue. (A) Central portion of medial meniscus was sectioned in the sagittal direction (red). The sagittal tissue portion was cut by a cryostat microtome at a thickness of 10 μm. The regions were defined by the purple line as the outer one-third, middle, and inner one-third of the tissue. (B) AFM cantilever tip with a glass microsphere attached (5 μm in diameter) was used to probe the tissue sample surface of the ECM. (C) Representative force maps (scan size of 20 μm × 20 μm) of outer, middle, and inner regions were generated from which the elastic moduli was extracted and plotted in histogram distributions.
