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. Author manuscript; available in PMC: 2015 Aug 20.
Published in final edited form as: Sci Transl Med. 2014 Aug 20;6(250):250ra115. doi: 10.1126/scitranslmed.3009569

Fig. 2. Denervation attenuates gastric tumor progression in mice.

Fig. 2

(A) Gross appearance of mouse stomachs at 18 months of age and representative micro-photographs of the histopatho-logical appearance of the corpus region of the anterior and posterior sides of the stomach from age-matchedINS-GAS mice (Control) and mice that underwent anterior UVT at 8 or 12 months of age. Scale bars, 100 μm. Red arrows, vagotomy side. (B) Volume density of tumor. Means ± SEM. Paired t test between the anterior and the posterior sides of the stomach: P = 0.589 (n = 21, Control), P = 2.56 × 10−5 (n = 17, UVT at 8months ofage),P=2.17×10−4 (n = 14, UVT at 10 months), P = 0.055 (n = 12, UVT at 12 months). (C)Pathological score for dysplasia. Means ± SEM. Paired t test between the anterior and the posterior sides of the stomach: P = 0.38 (n = 21, Control), P = 0.002 (n = 17, UVT at 8 months of age), P=0.047(n=14,UVT at 10 months), P=0.018(n=12,UVT at 12 months). (D) Kaplan-Meier curves showing survival of INS-GAS mice that underwentUVTat8(red),10(green), or 12 months of age (blue), or of age-matched INS-GAS mice (Control) (black). P = 0.01 between control and UVT groups at 8 months. (E) Proliferating cells in the anterior and posterior mucosa of the stomach of INS-GAS mice at 2 months after vagotomy and/or Botox injection. Means ± SEM. Paired t test was used to compare the anterior and posterior sides of the stomach. P = 0.291 (n = 6, Vehicle), P = 0.007 [n = 6, unilateral anterior Botox (UB)], P = 0.595 [n = 7, bilateral Botox (BB)], P = 0.326 [n = 7, bilateral Botox plus anterior UVT (BB + UVT)], P = 0.0007 (Vehicle anterior versus UB anterior, Dunnett's test). (F) Volume density of tumor in INS-GAS mice subjected to saline (intraperitoneally) (Control), UB + saline (intraperitoneally), UB + 5-fluorouracil (5-FU) + oxaliplatin (OXP) (intra peritoneally), or UVT + 5-FU + OXP (intraperitoneally). Means ± SEM. Paired t test was used to compare the anterior and posterior sides of the stomach. P = 0.172 (n = 10, Control), P = 0.200 (n = 10, UB + saline), P = 0.0004 (n = 24, UB + 5-FU + OXP), P = 0.006 (n = 16, UVT + 5-FU + OXP). (G) Gross appearance of representative stomachs from INS-GAS mice subjected to 5-FU + OXP with UB or UVT (reduced tumor burden indicated by arrows). (H) Kaplan-Meier curves showing survival of INS-GAS mice that underwent sham operation and 5-FU + OXP treatment (black), UB and 5-FU + OXP (red), or anterior UVT and 5-FU + OXP (blue). *P = 0.041, **P = 0.0069.