Table 1.
Summary of the histopathological and T2-weighted MR findings obtained in each rat 28 days after 15 min temporary MCA occlusion.
| tMCAo group | Subject | Histopathological findingsb |
T2-weighted MRIc | ||
|---|---|---|---|---|---|
| Selective neuronal loss (SNL) | Partial infarction | Complete infarction | |||
| 30 min | 1 | +SI(1–3), SBF(5), M(1, 2, 5), I(2–4), V(8), A(8) | ++ S2(3, 4, 6), SBF(4–6), I(2), A(7, 8), R(7) | ++ S1(2), SBF(3–6), S2(4, 5), R(5, 7), A_7 | ++ |
| 30 min | 2 | +S1(1, 2, 6), S2(6), SBF(4, 5), A(7, 8), Pa(7), V(8) | ++ S1(1, 2, 6), S2(3, 4), SBF(3–5), V(8) | ++ Sl(2, 6), SBF(3–5), S2(3, 4), 1(2) | ++ |
| 30 min | 3 | + Sl(2, 6), S2(3), SBF(4–5), Ml(l), A(7) | ++ M(1), S1(2, 3, 6), S2(3–5), SBF(4, 5), A(7), R(6, 7), V(8) | ++ S1(2, 3, 6), SBF(3–6), S2(3–6), R(6), A(7), I(5), V(8) | ++ |
| 22 min | 1 | + S1(1, 3, 4, 6), SBF(4, 5), M(1, 2), 1(2, 3), V(8), A(7, 8), R(6), Pa(7) | ++ Sl(l, 3), SBF(3–6), M(l, 2), I(2), S2(3–6), V(8), A(7, 8), Pa(7) | – | + |
| 22 min | 2a | – | – | – | – |
| 22 min | 3 | +S1(1, 2, 3, 6), SBF(4, 5), I(2, 4), S2(3–5), A(8), R(6), Pa(8) | ++ Sl(l, 2), SBF(3–6), S2(3–6), A(8), Pa(7) | – | + |
| 15 min | 1 | +Sl(2), SBF(4–6), I(2–4), S2(4, 6), R(5, 6), Pa(5), V(8), A(7, 8) | – | – | – |
| 15 min | 2 | +M(2), S1(2–6), SBF(3–6), I(2), S2(3–6), R(5–7), Pa(7), V(8), A(7, 8) | – | – | – |
| 15 min | 3 | + S1(2), I(3), R(5), V(8), A(8) | – | – | – |
| 15 min | 4 | + SBF(6), S2(6), R(6–8), A(7, 8) | – | – | – |
| 15 min | 5 | +M(2, 3), S1(2, 4, 6), SBF(3, 4, 6), I(2, 4), S2(3, 4, 6), A(6) | – | – | – |
| 15 min | 6 | + S1(2–5), SBF(3–6), I(2, 4), P(2), C(2), S2(3–6), Pa(7), V(8), A(6, 8) | – | – | – |
| 15 min | 7 | + S1(5), SBF(3, 6), I(3, 5), S2(3, 5, 6), R(6–7), A(6) | – | – | – |
Code: + = moderate; ++ = severe; - = absent.
aThis rat was found at post mortem to have unusual vascular anatomy of the MCA and no ischemic pathology and was excluded post hoc from further analysis.
bThe location of the ischemic changes is listed according to the ROI affected and in brackets the sections where this ROI was affected (see Materials and Methods for details). Region code: A–auditory cortex, C–caudate putamen, I–insular cortex, M–motor cortex, P–piriform cortex, Pa–parietal cortex, R–rhinal cortex, S1–primary somatosensory cortex, S2–secondary somatosensory cortex, SBF–primary somatosensory cortex barrel field, T–thalamus, V–visual cortex. Section 1 = Bregma +2.70 mm; Section 2 = Bregma +1.00 mm; Section 3 = Bregma -0.26 mm; Section 4 = Bregma -0.92 mm; Section 5 = Bregma -2.12 mm; Section 6 = Bregma -3.14 mm; Section 7 = Bregma -4.52 mm; Section 8 = Bregma -6.04 mm.
cFor SNL, no MRI changes could be identified.