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. Author manuscript; available in PMC: 2015 Mar 26.
Published in final edited form as: Cytometry A. 2013 Aug 8;83(10):898–912. doi: 10.1002/cyto.a.22340

Figure 2.

Figure 2

A: cAMP dose-dependence of FRET emission; B: the same data normalized to the CFP emission peak (473 nm); C: FRET response calculated using a one filter set method; D: FRET response calculated using a two filter set method; E: FRET response calculated using a three filter set method; F: FRET response calculated using a three filter set method and corrected for changes in CFP concentration. Note that panels C and E represent FRET indices, whereas panels D and F represent FRET efficiencies. Normalized FRET responses are shown in Figure 4. [Color figure can be viewed in the online issue, which is available at wileyonlinelibrary.com]