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. 2015 Mar 26;10(3):e0121625. doi: 10.1371/journal.pone.0121625

Table 1. Mean values (n = 45)1 and ANOVA 2 for score of iNOS, S100 and cytokeratin intensity of immunostaining 3 observed in the muscle tissue of pacus inoculated with BCG.

Period iNOS S100 Cytokeratin
3 2.77A 1.25D 0.00C
7 2.81A 1.40D 0.00C
14 2.51B 1.84C 1.73B
21 2.46B 2.48B 2.33AB
33 1.80C 2.73A 2.86A
F value 20.63 43.20 38.05
Pr>F 4 <.0001 <.0001 <.0001
CV 4 28.53 26.93 80.27

1 Mean values (n = 45: nine fish X five fields analyzed per animal) with at least one letter in common do not differ by the T test.

2 The analysis of variance within each parameter was represented by capital letters (columns) for comparison of data from each treatment through the experimental days.

3 Immunostaining score: 0 (without immunostaining), +1 (Weak = 10 to 20% of positive cells), +2 (Moderate = 20 to 50%) and +3 (Strong = ≥50%).

4 Pr>F: Probability of significance associated to the F value/ CV: Coefficient of variation.