Table 1. Oligonucleotide primers used in this study.
| Primers | Sequence 5’-3’ | Use | Reference or source |
|---|---|---|---|
| KAN-2 FP-1 | ACCTACAAAGCTCTCATCAACC | Identify Tn insertion | Epicentre Biotech. |
| KAN-2 RP-1 | GCAATGTA CATCAGAGATTTTGAG | Identify Tn insertion | Epicentre Biotech. |
| PD1671.F | GAATTCTTATTCAATTGGGGGTTACT | Complementation | This study |
| PD1671.R | GAATTCTCTTGTTTGAGTTTGCTATG | Complementation | This study |
| PD1671.RecF | CTTCAAGGTGCTGGCATACATG | RT-PCR a PD1671 REC domain | This study |
| PD1671.RecR | GAAATCATCGGCACCACTATCA | RT-PCR PD1671 REC domain | This study |
| PD1671.GgF | TATGGTTACACTGCTTTCGAGC | RT-PCR PD1671 GGDEF domain | This study |
| PD1671.GgR | TTTAAAGCCTTGATTCAGCGGG | RT-PCR PD1671 GGDEF domain | This study |
| PD1671.EalF | CGTTCATCCACATAAACGTAGC | RT-PCR PD1671 EAL domain | This study |
| PD1671.EalR | GTTTGCAAGACGCAGTTATTCA | RT-PCR PD1671 EAL domain | This study |
| XP1275.RT.F | TTATGTAAGCGTCTTGGTGTGG | RT-PCR dnaQ | This study |
| XP1275.RT.R | GCACATGACCAGCGATCTTAC | RT-PCR dnaQ | This study |
| XP1455.RT.R | GGTGTGTGCATTTGCTTCTATG | RT-PCR gumJ | This study |
| XP1455.RT.F | GAGGAGAGTGAGGAAGGGATCT | RT-PCR gumJ | This study |
| XP1460.RT.R | TCTTCCGTGTCTTGGGATTC | RT-PCR gumD | This study |
| XP1460.RT.F | AATGACAGGCACATGACCAA | RT-PCR gumD | This study |
| RST31 | GCGTTAATTTTCGAAGTGATTCGATTGC | Basipetal movement | [31] |
| RST33 | CACCATTCGTATCCCGGTG | Basipetal movement | [31] |
a RT-PCR—reverse transcriptase-polymerase chain reaction.