Table 2. Relative X. fastidiosa RNA levels.

Gene or domain a	Wild-type	PD1671 mutant	Complemented PD1671
PD1671 REC domain	1.11 ± 0.11	0.99 ± 0.07	1.78 ± 0.62
PD1671 GGDEF domain	0.96 ± 0.06	0.92 ± 0.18	1.20 ± 0.30
PD1671 EAL domain	0.96 ± 0.08	0.00 ± 0.00 b	1.40 ± 0.62
gumD	1.18 ± 0.45	2.86 ± 0.31 b	1.02 ± 0.01
gumJ	1.36 ± 0.33	2.61 ± 0.45 b	1.25 ± 0.22

^a RT-PCR (reverse transcriptase-polymerase chain reaction) experiments performed in Vitis vinifera xylem sap (three to six independent experiments with three replicates each). The standard deviations of the normalized means are shown. Expression of the gene regions was normalized to dnaQ gene expression [42]. Gene segments amplified: PD1671-REC domain (115 to 357bp), PD1671-GGDEF domain (601 to 858bp), PD1671-EAL domain (1325 to 1621bp), gumD (628–847bp), and gumJ (4–229bp).

^b Statistically significant compared to wild-type (P<0.01).