Table 3. Relative X. fastidiosa exoenzyme activity.
Extracellular enzyme a | Wild-type | PD1671 mutant | Complemented PD1671 |
---|---|---|---|
Carboxymethyl cellulase | 1.01 ± 0.20 | 1.10 ± 0.30 | 1.20 ± 0.25 |
Endo-β-1,4-mannanase | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
Polygalacturonase | 0.00 ± 0.00 | 0.00 ± 0.00 | 0.00 ± 0.00 |
Protease | 2.12 ± 0.20 | 2.20 ± 0.30 | 2.10 ± 0.25 |
a Extracellular enzyme activities were estimated from the diameter (mm) of the halo zones of supernatant enzymatic activity surrounding each well. All assays were performed three times, with five replicate plates each. The standard deviations of the means for each enzyme are shown.