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. 2014 Jul 15;14(4):387–398. doi: 10.1002/elsc.201400036

Figure 4.

Figure 4

Functional analysis of cell-free synthesized scFv fragments by ELISA. Cell-free synthesis was performed using the vesicle-containing insect cell-free expression system. scFv molecules were tested for binding to their peptide antigens SMAD2, SMAD2-P, and CXCR5. (A) Binding of Mel-SH527-IIA4 vs. SH527-IIA4, (B) binding of Mel-SH527-IIC10 vs. SH527-IIC10, and (C) binding of Mel-SH855-C11 vs. SH855-C11. Lysate fractions containing scFv fragments with melittin signal sequence (Mel-SH527-IIA4, Mel-SH527-IIC10, Mel-SH855-C11) show strong binding to their corresponding antigen, whereas scFv molecules without signal sequence (SH527-IIA4, SH527-IIC10, SH855-C11) only show weak binding or no binding at all. Error bars show SDs calculated from triplicate analysis (n = 3).