Figure 5.

Qualitative and quantitative analyses of cell-free synthesized and ¹⁴C-leucine-labeled scFv molecules. Cell-free synthesis was performed using a commercially available cell-free transcription–translation system based on E. coli cell extracts (EasyXpress Disulfide E. coli Kit, Qiagen). (A) Autoradiograph of ¹⁴C-leucine-labeled scFvs. (B) Diagram showing total protein yields of scFv antibody fragments with melittin signal sequence in the translation mixture (TM) and supernatant fraction (SN) after centrifugation. (C) Diagram showing total protein yields of scFv antibody fragments without melittin signal sequence in the TM and supernatant fraction (SN) after centrifugation. Error bars show SDs calculated from triplicate analysis (n = 3).