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. 2014 Jul 15;14(4):387–398. doi: 10.1002/elsc.201400036

Figure 6.

Figure 6

ELISA analysis of scFv molecules synthesized using the insect cell-free expression system or a commercially available E. coli cell-free expression system (EasyXpress Disulfide E. coli Kit, Qiagen). scFv molecules were tested for binding to their peptide antigens SMAD2, SMAD2-P, and CXCR5. (A) Binding of Mel-SH527-IIA4, (B) binding of Mel-SH527-IIC10, and (C) binding of Mel-SH855-C11. scFv molecules synthesized in insect cell lysate recognize their corresponding antigens with high specificity. Error bars show SDs calculated from triplicate analysis (n = 3).