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. Author manuscript; available in PMC: 2015 Mar 26.
Published in final edited form as: J Immunol. 2009 Mar 15;182(6):3380–3389. doi: 10.4049/jimmunol.0802598

FIGURE 1.

FIGURE 1

Stimulating splenocytes with anti-CD3ε plus anti-CD28 induces Notch-dependent perforin and granzyme B expression in CD8+ T cells. A, CD8+ T cells were isolated from splenocytes that were stimulated for 2 days and whole cell lysates were analyzed for Notch1 expression by immuno-blotting. Extracellular and transmembrane portions of Notch1 (top bands) and N1ICD (bottom bands) are shown. B, Isolated CD8+ T cells as in A were used for analysis of IFN-γ expression by intracellular staining and flow cytometry. The indicated days are stimulation periods with anti-CD3ε and anti-CD28. C, Parallel samples were used to determine the expression of perforin and granzyme B by real-time quantitative PCR and by intracellular staining (D) followed by flow cytometric analysis. For these experiments, stimulations were conducted for 1–3 days. Open histograms represent isotype controls. Data shown represent one of three independent replicates.

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